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KMID : 0984920150170010010
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Journal of Skin Barrier Research
2015 Volume.17 No. 1 p.10 ~ p.16
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Mass Spectrometry Based Proteomics and Its Applications for Skin Research
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Lee Ju-Yeon
Kim Jin-Young
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Abstract
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Peptide sequencing is the key step in commonly employed bottom-up approaches within mass spectrometry-based proteomics. Usually proteins are digested with enzymes such as trypsin followed by 1- or 2-dimensional chromatographic separation techniques prior to tandem MS analysis. The current method of choice for peptide identification is collision induced dissociation (CID). The development of alternative fragmentation techniques such as electron transfer dissociation (ETD) has extended the possibilities within tandem mass spectrometry. Recent advances in instrumentation allow peptide fragment ions to be detected with high speed and sensitivity at high resolution and high mass accuracy. Sequential windowed acquisition of all theoretical MS (SWATH-MS) method based on data independent acquisition (DIA) data analysis was described for the large scale proteome analysis. Owing to the nature of DIA data, which contain a complete record of all fragment ions of a biological sample, SWATH enables fast and reproducible conversion of a small amount of sample into a simple digital file representing the mass spectrometry-quantitative proteome of the sample
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KEYWORD
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spectrometry, Quantitative proteomics' SWATH
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